Effect of antioxidants and Kin in the Induction of shoots explants of the Australian finger lime (Citrus australasica) in vitro

Abstract

      Finger lime is a small-leaved citrus fruit native to Australia and increasingly increasingly popular worldwide, Finger lime is monoembryonic, initial studies to develop a tissue culture protocol for its propagation were unsuccessful due to complete leaf abscission during the culture Initiation stage, In this study, the effect of different concentrations of sodium hypochlorite (0, 15, 20, 25, 35, 45)% was studied to sterilize the nodal segment, and shoot tip, as the concentration of 20% for 15 minutes gave the lowest contamination rate and the best response rate, and shoot tip gave the lowest contamination rate and the single nodes gave the best response rate, while the black variety gave the lowest contamination rate and the highest response rate. The effect of different concentrations of Kin (0, 2, 4, 6, 8) mg L^-1 with the addition of 400 mg ^L-1 PVP or 1.5 mg L^-1 of activated charcoal was studied, as the concentration of 4 mg L^-1 of Kin gave the highest number of shoots, the longest length of shoots and the highest number of leaves, and PVP gave the highest response rate and the highest number of shoots The longest shoot length, the largest number of leaves, and the lowest percentage of yellowing and leaf abscission, while the black variety gave the highest response percentage, the highest number of shoots, the longest shoot length, the largest number of leaves, and the lowest percentage of yellowing and leaf abscission.