The Role of Cytokinin and IAA in the Propagation of Three Varieties of Finger Lime (Citrus Australasica) by Tissue Culture

Abstract

The Experiment was carried out in the plant tissue culture laboratory of the Department of Horticulture and Garden Engineering / College of Agriculture / University of Diyala, to propagate three varieties  of  Finger Lime (Citrus australasic) (Green, Red and Black) in vitro, during Initiation stage, the effect of different concentrations of Kin was studied. If the concentration of 4 mg L^-1 gave the highest response rate, the largest number and  length of shoots, the largest number of leaves, and the lowest percentage of yellowing and leaves drop , the red variety had the highest response rate while The black variety gave the highest number and length of shoots  and number of leaves and the lowest percentage of yellowing and leaves drop , During the multiplication stage, the effect of concentrations of  IAA  or BA was studied in combination with the best concentration of  Kin in  multiplying single node explants explant and planting them in MS medium. The results showed that after 6 weeks, the highest rate of number and length of shoots  and average number of while The black variety outperformed the other varieties by giving the highest number and length of shoots, number of leaves and the lowest percentage of yellowing and leaves drop, As for BA gave the highest concentration of 1.0 mg L^-1 length of shoots, while the concentration of 2.0 mg L^-1 gave the highest number of leaves, and the concentration of 3.0 mg L^-1, it gave the highest amount of callus formation,. As for the varieties, the black variety outperformed by giving the highest percentage of shoots, shoot length, number of leaves and the lowest percentage of yellowing and leaves drop, As for callus formation, the green variety gave the largest amount of callus formation.