Effect of Bovine Serum on Quorum Sensing Genes Expression in MDR Resistant P. Aeruginosa

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Noor M. Ghazi, Hameed M. Jasim

Abstract

Background: Pseudomonas aeruginosa, similar to several bacteria, use chemical signals for intercellular communication via use of quorum sensing (QS) mechanisms. QS enable the bacteria groups to detect population density and, in reaction to variations in cell density, to synchronize their behaviors. The current study intended to investigatethe effectof bovine serum on quorum sensing genes expression in Pseudomonas aeruginosaduring early stages of growth.


Methodology: Pseudomonas aeruginosa isolates were obtained from burns, wounds, urine, and ears. Bacteria isolates were identified in accordance to their biochemical reactions, then antibiotic susceptibility and The isolates' ability to build biofilms was examined, and each bacterial isolate's genomic DNA was extracted in order to find quorum sensing genes (rhlR, rhlI, lasR, and lasI).


Result: From different clinicalspecimens, twenty-five Pseudomonas aeruginosa isolates were identified,antibiotic resistance was present in these isolates., and it was found that only 10(40%) of these isolates were biofilm producers. Biofilm virulence genes lasR, lasI, rhlR, and rhlI were detected in all Pseudomonas aeruginosa isolates examined. 


Conclusion: lasR, lasI, rhlR, and rhlI genes were common inPseudomonas aeruginosa isolates that have high rate of resistance to all antibiotics, and strong ability in biofilm formation.

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